Enterolert 250

Accurately detects enterococci in 24 hours

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Overview

Easy
  • Ready-to-use media.
  • Simple procedure.
  • Unit-dosed packaging eliminates media preparation.
  • Reagent contains no toxic compounds.

Rapid
  • Confirmed results in 24 hours.
  • Simplified quality control.
  • Less than 1 minute of hands-on time.
Accurate 
  • More accurate when compared to ISO 7899-2 on all samples.
  • Liquid enrichment ensures optimised recovery.
  • Eliminates subjective interpretation of results determined by traditional methods.

Science

How the Enterolert 250 Test works

The Enterolert 250 Test uses proprietary Defined Substrate Technology (DST) nutrient indicator to detect enterococci. The Enterolert 250 Test provides confirmed presence/absence results in 24 hours. The Enterolert 250 Test utilises orth-nitrophenyl-ß-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background colour in its formulation.

When the substrate is metabolised by enterococci, the sample turns from blue to green to indicate detection. Any change from the original colour to green is considered a positive result. No ultraviolet light source is required. The Enterolert 250 Test detects enterococci at 1 cfu in 250 mL bottled water samples in 24 hours.

 

How to use

 

Learn how to use the Enterolert 250 Test


Presence/Absence


Step 1

Add reagent to sample, shake to mix and then incubate at 41 ± 1°C for 24 hours; results are valid for up to 28 hours of incubation.

Step 2

Blue indicates negative for enterococci. Green indicates positive for enterococci.

Frequently asked questions

 

The Enterolert 250 Test is designed to detect Enterococcus species in bottled water samples. It is based on IDEXX’s proprietary Defined Substrate Technology (DST). The Enterolert 250 Test utilizes ortho-nitrophenyl-ß-D-glucoside as a nutrient indicator and incorporates a specifically designed blue background colour in its formulation. When the enzyme substrate is metabolised by the enterococci, the sample turns from blue to green to indicate a positive detection. Any change from the original colour to green is considered a positive result. No ultraviolet light source is required. The Enterolert 250 Test detects enterococci at 1 cfu in 250 mL bottled water samples in 24 hours.

The Enterolert 250 Test is designed to detect enterococci in bottled water. As with any microbial method, some Enterococcus species are more easily detected than others. Some examples of Enterococcus species we have detected in trials and studies are:
Enterococcus faecalis
Enterococcus faecium
Enterococcus hirae
Enterococcus gallinarum
Enterococcus casseliflavus
Enterococcus durans
Enterococcus avium

There is no comparator for the Enterolert 250 Test. For comparison, use a negative control when interpreting results.

The Enterolert 250 Test is incubated at 41°C ± 1.0°C for 24 hours and the results are valid up to 28 hours, unless your local regulations indicate differently.

Yes, if the results are positive. An inoculated Enterolert 250 Test sample that is positive for enterococci before 24 hours is a confirmed positive test for enterococci.

Yes, if the results are negative. If an inoculated Enterolert 250 Test sample is inadvertently incubated over 28 hours, lack of green color is a valid negative test; a green colour after the 28 hours is not valid and should be repeated or verified.

When the enzyme substrate is metabolised by the enterococci, the sample turns from blue to green to indicate a positive result. Any change from the original colour to green is considered a positive result.

The Enterolert 250 Test is designed specifically for bottled water testing.

The Enterolert 250 reagent powder should be white to off-white in colour with a slight greenish tint; it should be dry and free-flowing. Any concerns about the colour or integrity of the reagent powder should be directed to IDEXX Technical Support.

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Product Information

Enterolert 250 Test (20-test pack)
Catalogue Number: WENT250-20

Water Customer Support

IDEXX Laboratories Canada Corp.
1345 Denison Street
Markham, Ontario
L3R 5V2

1-416-798-4988
1-800-667-3411

Corporate Headquarters
IDEXX Laboratories, Inc.
One IDEXX Drive
Westbrook, Maine 04092 USA

Tel: 1-800-321-0207
Fax: 1-207-556-4630

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